Product Description
Cat.#: T0001 Concn.: ~1mg/ml Mol.Wt.:
Size: Source: Mouse Clonality: Monoclonal
Application: WB 1:3000-1:10000, IF/ICC: 1:200, IP 1:200 *The optimal dilutions should be determined by the end user.
Reactivity: All
Storage: Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Purification: Affinity-chromatography.
Immunogen: A synthetic peptide EQKLISEEDL coupled to KLH.
Description: c-Myc-tag antibody is part of the Tag series of antibodies, the best quality in the research. The immunogen of c-Myc tag antibody is a synthetic peptide corresponding to residues 410-419 of the human p62 c-myc protein conjugated to KLH. C-Myc tag antibody is suitable for detecting the expression level of c-Myc or its fusion proteins where the c-Myc tag is terminal or internal.
Western blot analysis of C-Myc-Tag Mouse Monoclonal Ab expression in MYC-tag fusion protein sample
IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
For Research Use Only. Not for use in diagnostic and therapeutic procedures. Not for resale without express authorization.
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